2JLG

STRUCTURAL EXPLANATION FOR THE ROLE OF MN IN THE ACTIVITY OF PHI6 RNA-DEPENDENT RNA POLYMERASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 32
Unit cell lengths	109.026, 109.026, 158.835
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.944 - 2.800
R-factor	0.2196
Rwork	0.216
R-free	0.29470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hhs
RMSD bond length	0.000
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.210	1.000
Number of reflections	52552
<I/σ(I)>	5.9	1.2
Completeness [%]	99.7	100
Redundancy	4.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1