2JK4
Structure of the human voltage-dependent anion channel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-24 |
Detector | ADSC CCD |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 77.990, 77.990, 167.040 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 4.100 |
R-factor | 0.3314 |
Rwork | 0.325 |
R-free | 0.38730 |
Structure solution method | OTHER |
Starting model (for MR) | NONE |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHELXD (SOLOMON SHARP) |
Refinement software | BUSTER-TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.000 | 4.300 |
High resolution limit [Å] | 4.100 | 4.100 |
Rmerge | 0.100 | 0.540 |
Number of reflections | 4887 | |
<I/σ(I)> | 16.4 | 3.5 |
Completeness [%] | 99.5 | 99.1 |
Redundancy | 16.8 | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.2 M MAGNESIUM CHLORIDE HEXAHYDRATE, 0.1 M HEPES SODIUM PH 7.5, 30% V/V POLYETHYLENE GLYCOL 400 |