2JJT

Structure of human CD47 in complex with human signal regulatory protein (SIRP) alpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-04
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	53.620, 63.720, 123.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.810 - 2.300
R-factor	0.238
Rwork	0.235
R-free	0.28800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2JJS CHAINS A AND C
RMSD bond length	0.008
RMSD bond angle	1.193
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.450
High resolution limit [Å]	2.300	2.300
Rmerge	0.190	0.570
Number of reflections	19339
<I/σ(I)>	7.4	3.9
Completeness [%]	99.5	98.5
Redundancy	7	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5	278	100 NL SIRP ALPHA / CD47 COMPLEX (1:1 RATIO, EACH PROTEIN AT APPROX. 0.375 UM) PLUS 100 NL RESERVOIR (0.1 M CITRATE PH 5.0, 30% W/V PEG 3000) EQUILIBRATED AGAINST 95 UL OF RESERVOIR AT 5 C.