2JHJ
3-methyladenine dna-glycosylase from Archaeoglobus fulgidus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-01-13 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.890, 48.850, 104.460 |
| Unit cell angles | 90.00, 106.07, 90.00 |
Refinement procedure
| Resolution | 12.000 - 1.900 |
| R-factor | 0.184 |
| Rwork | 0.181 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jhn |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.366 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.430 |
| Number of reflections | 53742 | |
| <I/σ(I)> | 11.4 | 2.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M BICINE, PH9.0 10% W/V PEG20000 2% V/V DIOXANE |
