2JHN

3-methyladenine dna-glycosylase from Archaeoglobus fulgidus

Summary for 2JHN

• Entry DOI: 10.2210/pdb2jhn/pdb
• Related: 2JHJ
• Descriptor: 3-METHYLADENINE DNA-GLYCOSYLASE, MERCURIBENZOIC ACID, MERCURY (II) ION, ... (7 entities in total)
• Functional Keywords: alka, dna repair, n1-methyladenine, n3-methylcytosine, hyperthermophiles, archaeoglobus fulgidus, hydrolase
• Biological source: ARCHAEOGLOBUS FULGIDUS
• Total number of polymer chains: 2
• Total formula weight: 70848.91

Authors

Leiros, I.,Nabong, M.P.,Grosvik, K.,Ringvoll, J.,Haugland, G.T.,Uldal, L.,Reite, K.,Olsbu, I.K.,Knaevelsrud, I.,Moe, E.,Andersen, O.A.,Birkeland, N.K.,Ruoff, P.,Klungland, A.,Bjelland, S. (deposition date: 2007-02-22, release date: 2007-04-10, Last modification date: 2024-05-08)

Primary citation

Leiros, I.,Nabong, M.P.,Grosvik, K.,Ringvoll, J.,Haugland, G.T.,Uldal, L.,Reite, K.,Olsbu, I.K.,Knaevelsrud, I.,Moe, E.,Andersen, O.A.,Birkeland, N.K.,Ruoff, P.,Klungland, A.,Bjelland, S.
Structural Basis for Enzymatic Excision of N1-Methyladenine and N3-Methylcytosine from DNA
Embo J., 26:2206-, 2007

PubMed Abstract: N(1)-methyladenine (m(1)A) and N(3)-methylcytosine (m(3)C) are major toxic and mutagenic lesions induced by alkylation in single-stranded DNA. In bacteria and mammals, m(1)A and m(3)C were recently shown to be repaired by AlkB-mediated oxidative demethylation, a direct DNA damage reversal mechanism. No AlkB gene homologues have been identified in Archaea. We report that m(1)A and m(3)C are repaired by the AfAlkA base excision repair glycosylase of Archaeoglobus fulgidus, suggesting a different repair mechanism for these lesions in the third domain of life. In addition, AfAlkA was found to effect a robust excision of 1,N(6)-ethenoadenine. We present a high-resolution crystal structure of AfAlkA, which, together with the characterization of several site-directed mutants, forms a molecular rationalization for the newly discovered base excision activity.

PubMed: 17396151
DOI: 10.1038/SJ.EMBOJ.7601662

Experimental method

X-RAY DIFFRACTION (1.8 Å)