2JGA
Crystal structure of human cytosolic 5'-nucleotidase III in complex with phosphate and magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-22 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 87.459, 101.036, 77.046 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.190 - 3.010 |
| R-factor | 0.185 |
| Rwork | 0.181 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cn1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.361 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0021) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.150 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.110 | 0.420 |
| Number of reflections | 7057 | |
| <I/σ(I)> | 24.1 | 6.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.4 | 13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 24% PEG1500 AND 20% GLYCEROL |
