2JFK
Structure of the MAT domain of human FAS with malonyl-CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-19 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 82.917, 91.410, 261.609 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.960 - 2.400 |
| R-factor | 0.224 |
| Rwork | 0.222 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jfd |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.415 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.070 | 0.610 |
| Number of reflections | 78546 | |
| <I/σ(I)> | 17.3 | 2.62 |
| Completeness [%] | 99.0 | 92.4 |
| Redundancy | 7.22 | 6.13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 16% PEG10K 0.16M MGCL2 0.08 M TRIS PH=8.5, pH 8.50 |
