2JF1
CRYSTAL STRUCTURE OF THE FILAMIN A REPEAT 21 COMPLEXED WITH THE INTEGRIN BETA2 CYTOPLASMIC TAIL PEPTIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-25 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 78.720, 78.720, 78.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.450 - 2.200 |
| R-factor | 0.25 |
| Rwork | 0.247 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2brq |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.888 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.460 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 | 0.460 |
| Number of reflections | 8482 | |
| <I/σ(I)> | 25.4 | 6.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 14 | 14.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 1.6-1.58 M AMMONIUM SULPHATE, 0.1 M NA-ACETATE PH 4.6 |
