2JB5
Fab fragment in complex with small molecule hapten, crystal form-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-30 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 71.949, 98.480, 153.782 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.700 - 2.800 |
| Rwork | 0.211 |
| R-free | 0.27150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vge |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNX (2005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.700 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.100 | 0.550 |
| Number of reflections | 13432 | |
| <I/σ(I)> | 17.1 | 2.2 |
| Completeness [%] | 97.6 | 79.8 |
| Redundancy | 5.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 2.3M AMMONIUM SULPHATE, 5% PEG400, 0.1M SODIUM CITRATE PH 4.0, FOR CRYO BUFFER ADITIONAL 15% GLYCEROL |
