2J6W
R164N mutant of the RUNX1 Runt domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-18 |
| Detector | BUILT IN ANL |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.948, 46.497, 63.552 |
| Unit cell angles | 90.00, 90.66, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.214 |
| Rwork | 0.209 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eao |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.238 |
| Data reduction software | HKL |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.120 | 0.390 |
| Number of reflections | 7962 | |
| <I/σ(I)> | 19.6 | 2.3 |
| Completeness [%] | 94.5 | 73.9 |
| Redundancy | 4.5 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 22% PEG 8000, 0.1M SODIUM CACODYLATE PH=6.5, 0.2M AMMONIUM SULFATE |
