2J1Y
Human p53 core domain mutant M133L-V203A-N239Y-G245S-N268D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.514, 73.076, 84.760 |
| Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
| Resolution | 37.400 - 1.690 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uol |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.400 | 1.780 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.070 | 0.270 |
| Number of reflections | 93315 | |
| <I/σ(I)> | 15.4 | 4 |
| Completeness [%] | 99.6 | 98.4 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
