2J0M
Crystal structure a two-chain complex between the FERM and kinase domains of focal adhesion kinase.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-26 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 67.490, 90.600, 242.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.390 - 2.800 |
| R-factor | 0.224 |
| Rwork | 0.221 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1MP8 AND 2AEH |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.304 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.100 | 0.550 |
| Number of reflections | 17515 | |
| <I/σ(I)> | 11.5 | 2.73 |
| Completeness [%] | 93.3 | 94.9 |
| Redundancy | 4.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 16% PEG20K, 250MM NACL, 100MM TRIS PH8.5, 10MM TCEP, pH 8.50 |
