2IVT

Crystal structure of phosphorylated RET tyrosine kinase domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-25
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	72.131, 70.848, 78.916
Unit cell angles	90.00, 101.13, 90.00

Refinement procedure

Resolution	50.000 - 2.600
R-factor	0.187
Rwork	0.184
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gjo
RMSD bond length	0.017
RMSD bond angle	1.689
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.500	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.050	0.130
Number of reflections	12129
<I/σ(I)>	12.4	5.1
Completeness [%]	100.0	100
Redundancy	3.6	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	PROTEIN 3 MG/ML IN 20 MM TRIS-HCL PH 8.0, 100MM NACL, 1MM DTT RESERVOIR 2.0 M SODIUM FORMATE, 0.1M SODIUM CITRATE PH 5.5,2.5MM ATP 5MM MAGNESIUM CHLORIDE VAPOUR DIFFUSION, SITTING DROP, 295 K