2IVE
Structure of protoporphyrinogen oxidase from Myxococcus xanthus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 148.566, 148.566, 132.745 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.330 - 2.700 |
| R-factor | 0.248 |
| Rwork | 0.247 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sez |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.122 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.300 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.140 | 0.570 |
| Number of reflections | 41382 | |
| <I/σ(I)> | 14.7 | 2.6 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M TRIS/HCL PH 7.5, 1.5M AMMONIUM SULPHATE, 20% GLYCEROL, 1% PEG 4000 |
