2IUX
Human tACE mutant g1234
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.436, 85.149, 133.424 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.800 |
R-factor | 0.2006 |
Rwork | 0.201 |
R-free | 0.23690 |
Structure solution method | OTHER |
RMSD bond length | 0.010 |
RMSD bond angle | 1.460 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.110 | 0.340 |
Number of reflections | 13572 | |
<I/σ(I)> | 7.7 | 2.8 |
Completeness [%] | 83.6 | 80.4 |
Redundancy | 39 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.7 | 0.1M SODIUM ACETATE PH4.7 15% PEG 4000, 10UM ZINC SULPHATE, pH 4.70 |