2IUI

Crystal structure of the PI3-kinase p85 N-terminal SH2 domain in complex with PDGFR phosphotyrosyl peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	278
Spacegroup name	P 21 21 21
Unit cell lengths	40.760, 61.940, 108.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.400
R-factor	0.1784
Rwork	0.178
R-free	0.24840
Structure solution method	OTHER
RMSD bond length	0.009
RMSD bond angle	1.650
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.060	0.310
Number of reflections	12930
<I/σ(I)>	23.37	4.92
Completeness [%]	98.2	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.1M SODIUM ACETATE PH 4.6, 0.2M AMMONIUM ACETATE, 30% PEG4000