2ISI
Crystal structure of Ape1 from Homo sapiens in a new crystal form complexed with a ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-06 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 167.347, 92.392, 94.006 |
| Unit cell angles | 90.00, 121.38, 90.00 |
Refinement procedure
| Resolution | 32.130 - 2.760 |
| Rwork | 0.228 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hd7 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.970 |
| High resolution limit [Å] | 2.760 | 2.860 |
| Rmerge | 0.060 | 0.440 |
| Number of reflections | 27372 | |
| <I/σ(I)> | 7.3 | 1 |
| Completeness [%] | 90.0 | 68.8 |
| Redundancy | 3.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 277 | 0.1M hepes, 70% MPD, pH 7.5, VAPOR DIFFUSION, temperature 277K |
