2IRF
CRYSTAL STRUCTURE OF AN IRF-2/DNA COMPLEX.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-08-15 |
Detector | RIGAKU |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 132.300, 132.300, 296.500 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 2.200 |
R-factor | 0.202 |
Rwork | 0.202 |
R-free | 0.24300 |
Structure solution method | MIRAS |
RMSD bond length | 0.010 |
RMSD bond angle | 18.500 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.200 |
High resolution limit [Å] | 2.200 | 2.100 |
Rmerge | 0.062 * | |
Total number of observations | 2494554 * | |
Number of reflections | 69536 * | |
Completeness [%] | 88.4 | 77.1 * |
Redundancy | 35.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 5.8 | 4 * | 50 MM MES-K (PH 5.8), 50 MM KCL, 4% 2-MPD,, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MES | ||
2 | 1 | 1 | KCL | ||
3 | 1 | 1 | MPD | ||
4 | 1 | 2 | MES | ||
5 | 1 | 2 | KCL | ||
6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | Mes-K | 50 (mM) | |
2 | 1 | reservoir | 50 (mM) | ||
3 | 1 | reservoir | 4 (%) | ||
4 | 1 | drop | protein | ||
5 | 1 | drop | 13mer DNA |