2IPL
Crystal structure of a disulfide mutant glucose binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-11 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.840, 36.680, 79.070 |
| Unit cell angles | 90.00, 124.03, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.200 |
| R-factor | 0.168 |
| Rwork | 0.167 |
| R-free | 0.19100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hph |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.196 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.300 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.054 | 0.262 |
| Number of reflections | 81424 | |
| <I/σ(I)> | 13.17 | 3.7 |
| Completeness [%] | 91.6 | 72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 0.1M HEPES, pH7.5, 20% PEG 1500, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
