2IPI
Crystal Structure of Aclacinomycin Oxidoreductase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-02-24 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.8423, 0.9793, 0.9797, 0.9392 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.500, 266.200, 68.700 |
| Unit cell angles | 90.00, 119.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.650 |
| R-factor | 0.185 |
| R-free | 0.24200 |
| Structure solution method | MAD |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE (2.09) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.700 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 246742 | |
| <I/σ(I)> | 11.5 | 3.4 |
| Completeness [%] | 95.9 | 57.3 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 293 | 20%(w/v)PEG6000, 0.2M ammonium chloride, 0.1M Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 8.00 |
