2IP4

Crystal Structure of Glycinamide Ribonucleotide Synthetase from Thermus thermophilus HB8

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-06
Detector	MARRESEARCH
Wavelength(s)	0.9843
Spacegroup name	P 21 21 21
Unit cell lengths	72.308, 98.068, 121.142
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.000 - 2.800
R-factor	0.225
Rwork	0.218
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gso
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.100
Number of reflections	29262
Completeness [%]	95.6	96.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.2M ammonium sulfate, 0.1M MES, 30% PEGMME5000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K