2IOB

E. coli Bifunctional glutathionylspermidine synthetase/amidase Apo protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL12B2
Synchrotron site	SPring-8
Beamline	BL12B2
Temperature [K]	200
Detector technology	CCD
Collection date	2004-11-11
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	149.267, 92.964, 108.296
Unit cell angles	90.00, 109.37, 90.00

Refinement procedure

Resolution	50.000 - 2.200
Rwork	0.175
R-free	0.23600
Structure solution method	MAD
RMSD bond length	0.020
RMSD bond angle	1.929
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	XTALVIEW

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Rmerge	0.076
Number of reflections	70953
<I/σ(I)>	18.8
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	12% PEG 3350, 0.6M magnesium chloride, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	12% PEG 3350, 0.6M magnesium chloride, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K