2ILY

Crystal structure of poliovirus polymerase complexed with ATP and Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 65
Unit cell lengths	128.526, 128.526, 112.891
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.800 - 2.600
Rwork	0.230
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ra6
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	31801
<I/σ(I)>	11.9	3.7
Completeness [%]	97.6	96.3
Redundancy	5.31	5.02

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	sodium acetate, cacodylate, dtt, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K