2IL1
Crystal structure of a predicted human GTPase in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 45.463, 45.463, 315.454 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| Rwork | 0.241 |
| R-free | 0.26610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g17 chain A |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.415 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.052 | 0.039 | 0.430 |
| Number of reflections | 13543 | 1672 | 730 |
| <I/σ(I)> | 13.1 | ||
| Completeness [%] | 94.2 | ||
| Redundancy | 13.9 | 14.3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 22% PEG4000, 0.2M calcium acetate, 0.1M sodium cacodylate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
