2IJD
Crystal Structure of the Poliovirus Precursor Protein 3CD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-02-26 |
| Detector | RIGAKU |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 208.537, 230.436, 151.035 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.900 - 3.400 |
| R-factor | 0.20073 |
| Rwork | 0.199 |
| R-free | 0.23057 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l1n PDB ENTRY 1RA6 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.938 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.580 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Number of reflections | 49539 | |
| Redundancy | 5 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2 M ammonium sulfate, 0.1 M HEPES, 0.3% Jeffamine M600, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
