2IFG
Structure of the extracellular segment of human TRKA in complex with nerve growth factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-15 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 110.215, 81.681, 115.748 |
| Unit cell angles | 90.00, 104.15, 90.00 |
Refinement procedure
| Resolution | 12.000 - 3.400 |
| R-factor | 0.297 |
| Rwork | 0.297 |
| R-free | 0.33100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1www |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.500 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Number of reflections | 26668 | |
| Completeness [%] | 99.0 | 97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PEG, GLYCINE, PH 7.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K, pH 7.50 |
