2IDB
Crystal Structure of 3-octaprenyl-4-hydroxybenzoate decarboxylase (UbiD) from Escherichia coli, Northeast Structural Genomics Target ER459.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97894 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 194.295, 194.295, 106.051 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.200 - 2.900 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.26500 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.200 | 3.020 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.110 | 0.586 |
| Number of reflections | 85887 | |
| <I/σ(I)> | 17.9 | 4.9 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 8.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 20mM Tris, 20% PEG3350, 200mM ammonium tartrate, and 5mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
