2ID5

Crystal Structure of the Lingo-1 Ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	298
Detector technology	CCD
Collection date	2005-10-10
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	201.517, 149.720, 157.486
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	158.110 - 2.698
R-factor	0.216
Rwork	0.214
R-free	0.25500
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.301
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	158.110	2.800
High resolution limit [Å]	2.698	2.698
Number of reflections	122982
<I/σ(I)>	61	1.3
Completeness [%]	98.9	94.7
Redundancy	8	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291	1.4 M Am Sulfate, O.1 M Sodium Citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K