2IBF
Human vinculin's head domain (Vh1, residues 1-258) in complex with two vinculin binding sites of Shigella flexneri's IpaA (residues 565-587)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 154.478, 154.478, 154.478 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 109.110 - 3.200 |
| R-factor | 0.2452 |
| Rwork | 0.243 |
| R-free | 0.26970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.903 |
| Refinement software | BUSTER-TNT (1.3.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 109.110 | |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 10911 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
