2I9B
Crystal structure of ATF-urokinase receptor complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-07 |
Detector | MAR CCD 225 mm |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.914, 281.921, 62.811 |
Unit cell angles | 90.00, 105.41, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.800 |
R-factor | 0.22372 |
Rwork | 0.222 |
R-free | 0.26498 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ywh 2i9a |
RMSD bond length | 0.012 |
RMSD bond angle | 1.888 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 47048 | |
<I/σ(I)> | 17.6 | 2.44 |
Completeness [%] | 91.2 | 51.3 |
Redundancy | 2.8 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 22.5% w/v PEG3350, 200 mM ammonium sulfate, 100 mM Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |