2I4T
Crystal structure of Purine Nucleoside Phosphorylase from Trichomonas vaginalis with Imm-A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-16 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 62 |
| Unit cell lengths | 155.765, 155.765, 102.612 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.990 - 2.740 |
| R-factor | 0.17 |
| Rwork | 0.170 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.242 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.850 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmerge | 0.090 | 0.534 |
| Number of reflections | 37032 | |
| <I/σ(I)> | 9.9 | |
| Completeness [%] | 99.3 | 99.9 |
| Redundancy | 6.2 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 298 | 0.2 M Lithium Citrate, 30% PEG 3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 8.50 |
