2I35
Crystal structure of rhombohedral crystal form of ground-state rhodopsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2004-06-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 277.534, 277.534, 66.321 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 3.800 |
| R-factor | 0.37542 |
| Rwork | 0.373 |
| R-free | 0.41841 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hzx |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.437 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.940 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.092 | |
| Number of reflections | 8204 | |
| <I/σ(I)> | 22.3 | 1.3 |
| Completeness [%] | 84.5 | 59.2 |
| Redundancy | 15.9 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.3 | 278 | 80 mM MES, pH 6.3, 110 mM N-nonyl-beta-D-glucoside, 200 mM zinc acetate, 16 mM beta-mercaptoethanol, 0.4% Merpol DA, 0.1% sodium azide, equilibrated against 3.0 M ammonium sulfate, VAPOR DIFFUSION, temperature 278K |
