2I1Y
Crystal structure of the phosphatase domain of human PTP IA-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.93971 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.907, 74.229, 121.067 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.730 - 2.230 |
| R-factor | 0.20123 |
| Rwork | 0.199 |
| R-free | 0.26698 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fh7 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.146 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.225 | |
| Number of reflections | 32726 | |
| <I/σ(I)> | 2.7 | 0.5 |
| Completeness [%] | 98.7 | 98.7 |
| Redundancy | 6.8 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 294 | 25% PEG3350, 100 MM BIS-TRIS, 200 MM AMMONIUM ACETATE, PH 6.0, 10% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
