2I0Q
Crystal structure of a telomere single-strand DNA-protein complex from O. nova with full-length alpha and beta telomere proteins
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.11587 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 93.331, 93.331, 423.779 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.910 |
R-factor | 0.245 |
Rwork | 0.243 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jb7 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.280 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.980 |
High resolution limit [Å] | 1.910 | 1.910 |
Number of reflections | 85120 | |
<I/σ(I)> | 38.8 | 3.5 |
Completeness [%] | 98.9 | 95.7 |
Redundancy | 8.1 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.75 | 277.15 | 21% (w/v) PEG-4000, 10% (v/v) ethylene glycol, 1.5 M sodium chloride, 40 mM MES, 2 mM DTT, 0.02% sodium azide, VAPOR DIFFUSION, HANGING DROP, pH 5.75, temperature 277.15K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG-4000 | ||
10 | 1 | 2 | sodium azide | ||
11 | 1 | 2 | H2O | ||
2 | 1 | 1 | ethylene glycol | ||
3 | 1 | 1 | sodium chloride | ||
4 | 1 | 1 | MES | ||
5 | 1 | 1 | DTT | ||
6 | 1 | 1 | sodium azide | ||
7 | 1 | 1 | H2O | ||
8 | 1 | 2 | PEG-4000 | ||
9 | 1 | 2 | sodium chloride |