2HZY
Mouse fumarylacetoacetate hydrolase complexes with a transition-state mimic of the complete substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-01 |
| Detector | APS-1 |
| Wavelength(s) | 0.98000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.144, 109.472, 67.491 |
| Unit cell angles | 90.00, 102.35, 90.00 |
Refinement procedure
| Resolution | 24.140 - 1.350 |
| R-factor | 0.16901 |
| Rwork | 0.168 |
| R-free | 0.18815 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qcn |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.787 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 188128 | |
| <I/σ(I)> | 19.9 | 2.6 |
| Completeness [%] | 94.6 | 79.7 |
| Redundancy | 4.37 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M Sodium cacodylate, 0.3 M Sodium acetate, 30% (w/v) PEG 400, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
