2HZK
Crystal structures of a sodium-alpha-keto acid binding subunit from a TRAP transporter in its open form
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97940,0.97960,0.97565 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 104.505, 63.826, 127.897 |
| Unit cell angles | 90.00, 106.58, 90.00 |
Refinement procedure
| Resolution | 60.000 - 1.700 |
| R-factor | 0.18 |
| Rwork | 0.179 |
| R-free | 0.20600 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.309 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE (2.08) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 1.710 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 175128 | |
| Completeness [%] | 99.0 | 95.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293 | 100 mM sodium citrate 1.5M ammonium sulfate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
