2HYI
Structure of the human exon junction complex with a trapped DEAD-box helicase bound to RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-02 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9464 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 185.830, 88.260, 145.770 |
| Unit cell angles | 90.00, 110.77, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.213 |
| Rwork | 0.212 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 97913 | |
| <I/σ(I)> | 14.24 | 3.22 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4.75 | 4.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.8 | 297 | 7% PEG3350 50 mM Tris 200 mM NaAcetate, pH 8.8, VAPOR DIFFUSION, temperature 297K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG3350 | ||
| 2 | 1 | 1 | Tris | ||
| 3 | 1 | 1 | NaAcetate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | PEG3350 | ||
| 6 | 1 | 2 | NaAcetate | ||
| 7 | 1 | 2 | H2O |
