2HVL
Crystal structure of the HePTP catalytic domain C270S mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 61 |
| Unit cell lengths | 127.106, 127.106, 59.999 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.18924 |
| Rwork | 0.187 |
| R-free | 0.22769 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.310 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC ((FS)) |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 21632 | |
| Completeness [%] | 99.1 | 98.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 277 | Sodium/Potassium phosphate, acetate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
