2HT4
Structure of the Escherichia coli ClC chloride channel Y445W mutant and Fab complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-07 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9193 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 234.007, 95.940, 175.327 |
Unit cell angles | 90.00, 133.20, 90.00 |
Refinement procedure
Resolution | 40.000 - 3.200 |
R-factor | 0.24049 |
Rwork | 0.239 |
R-free | 0.26106 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.589 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.400 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.088 | |
Number of reflections | 45398 | |
Completeness [%] | 96.9 | 94.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 28% peg 300 v/v, 50 mM HEPES, 100mM NaBr, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |