2HSQ
Human vinculin (head domain, Vh1, residues 1-258) in complex with Shigella's IpaA vinculin binding site 2 (residues 565-587)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 203.170, 203.170, 203.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.000 - 3.970 |
| R-factor | 0.289 |
| Rwork | 0.286 |
| R-free | 0.31620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.445 |
| Refinement software | BUSTER-TNT (1.3.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 58.720 |
| High resolution limit [Å] | 3.970 |
| Number of reflections | 6410 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | ||||
| 1 |
