2HRE
Structure of human ferrochelatase variant E343K with protoporphyrin IX bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2005-12-15 |
| Detector | SIEMENS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.952, 88.388, 93.253 |
| Unit cell angles | 102.41, 109.34, 105.58 |
Refinement procedure
| Resolution | 43.660 - 2.500 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hrk |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.072 | 0.281 |
| Number of reflections | 53845 | |
| <I/σ(I)> | 13.5 | 3.4 |
| Completeness [%] | 96.7 | 93.5 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1 M Bis-Tris, 0.2 M Magnesium Chloride, 25% PEG 3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
