2HR7
Insulin receptor (domains 1-3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-07-12 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 103.861, 130.241, 160.924 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.320 |
| R-factor | 0.197 |
| Rwork | 0.195 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1igr |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.812 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.072 | 0.770 |
| Number of reflections | 94059 | |
| <I/σ(I)> | 12 | 2.1 |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 3.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 291 | 1.5-1.65 M ammonium sulfate, 2 % PEG 400, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K, pH 8.50 |
