2HNL
Structure of the prostaglandin D synthase from the parasitic nematode Onchocerca volvulus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Collection date | 2004-12-22 |
| Wavelength(s) | 0.803 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.783, 90.995, 106.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.184 |
| Rwork | 0.181 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 1PD2 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.716 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.069 | 0.031 | 0.360 |
| Number of reflections | 34249 | ||
| <I/σ(I)> | 15.1 | ||
| Completeness [%] | 99.7 | 99 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | Reservoir: 22% PEG4000, 10% Isopropanol, 0.1M Hepes pH 7.5 Protein stock: 5.5mg/ml in 100mM Na-acetate-trihydrate pH 8.0, temperature 298K, VAPOR DIFFUSION, HANGING DROP |
