2HJH
Crystal Structure of the Sir2 deacetylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.353, 89.566, 94.510 |
| Unit cell angles | 90.00, 104.95, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.850 |
| Rwork | 0.171 |
| R-free | 0.21500 |
| Structure solution method | SAD |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.124 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.09) |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.049 | 0.432 |
| Number of reflections | 71108 | |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 98.8 | 90.1 |
| Redundancy | 4.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 10% PEG 6000, 100mM TRIS pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
