2HI9
Crystal Structure of human native protein C inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.488 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 198.590, 70.950, 123.880 |
Unit cell angles | 90.00, 127.92, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.227 |
Rwork | 0.227 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | PHASER |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.083 | 0.396 |
Number of reflections | 60523 | |
<I/σ(I)> | 2.9 | |
Completeness [%] | 99.7 | 99 |
Redundancy | 3.9 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 296 | 66.7mM tri-potassium citrate, 6.7% PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |