2HHP
Structure of yeast poly(A) polymerase in a closed conformation.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.483, 84.918, 67.721 |
| Unit cell angles | 90.00, 111.78, 90.00 |
Refinement procedure
| Resolution | 33.040 - 1.800 |
| R-factor | 0.19849 |
| Rwork | 0.197 |
| R-free | 0.23551 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fa0 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.564 |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.448 | |
| Number of reflections | 56901 | |
| <I/σ(I)> | 46 | 3.46 |
| Completeness [%] | 99.7 | 98.3 |
| Redundancy | 6.9 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | The crystals were grown by combining the protein at 20mg/ml with an equal volume of 20% isopropanol, 20% Peg 4000, 100mM Citrate pH 5.6, and 0.1% beta mercaptoethanol, and then leaving the hanging drops for two weeks over an empty reservoir well., vapor diffusion, hanging drop |
