2HFZ
Crystal structure of RNA dependent RNA polymerase domain from West Nile virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 73.333, 103.449, 190.074 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 95.350 - 3.000 |
| R-factor | 0.26 |
| Rwork | 0.260 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hcs |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | BUSTER-TNT (1.3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.770 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.337 | |
| Number of reflections | 14328 | |
| <I/σ(I)> | 12.9 | 3.4 |
| Completeness [%] | 96.4 | 100 |
| Redundancy | 4.7 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | 5-25% PEG 1000 0.1 M NaCacodylate 0.3 M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.00 |
