2HF3
Crystal structure of monomeric Actin in the ADP bound state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-04-08 |
Detector | MAR scanner 345 mm plate |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 199.700, 54.070, 39.590 |
Unit cell angles | 90.00, 93.16, 90.00 |
Refinement procedure
Resolution | 19.190 - 1.800 |
R-factor | 0.175 |
Rwork | 0.175 |
R-free | 0.21300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j6z |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.080 | 0.390 |
Number of reflections | 38620 | |
<I/σ(I)> | 15.1 | 2.1 |
Completeness [%] | 98.4 | 86.8 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.4 | 277 | 25% MPD, 0.1M NaAcetate, 0.1M NaCl, 0.02M CaCl2, VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 5.40 |