2HEY
Crystal structure of murine OX40L bound to human OX40
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-21 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9330 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 104.729, 104.729, 478.161 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.2232 |
| Rwork | 0.221 |
| R-free | 0.24713 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | murine OX40L |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.041 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 68124 | |
| <I/σ(I)> | 12.2 | 2.1 |
| Completeness [%] | 98.6 | 96.4 |
| Redundancy | 4.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 0.1 M NaCl, 1.4 M Ammonium Sulfate, 0.1 M Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
