2HE0
Crystal structure of a human Notch1 ankyrin domain mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9769 |
| Spacegroup name | P 65 |
| Unit cell lengths | 96.982, 96.982, 108.719 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.510 - 1.900 |
| R-factor | 0.18998 |
| Rwork | 0.188 |
| R-free | 0.23094 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yyh |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.491 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.066 | 0.488 |
| Number of reflections | 45547 | |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.55M Sodium/Potassium Tartrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
